Advances and Clinical Practice in Pyrosequencing [Paperback]

The aim of this book is to improve pyrosequencing protocols as well as instrumentation for better clinical use by describing improvements and novel applications of pyrosequencing technology.  Divided into five parts, the books thirty chapters explore advances in pyrosequencing template preparation, pyrosequencing technology innovations, multiplex pyrosequencing based on barcodes, the miniaturization of pyrosequencing equipment, as well as various applications. As part of the Springer Protocols program, chapters contain the kind of detail and practical implementation advice to guarantee successful results in the lab.

Comprehensive and thorough, Advances and Clinical Practice in Pyrosequencing serves as a valuable reference for researchers who are engaged in personalized medicine, disease control, and DNA diagnosis in numerous other fields.

Part I: Template Preparation

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1. Preparation of Single-Stranded DNA for Pyrosequencing by LATE-PCR

??????????? Huiyong Yang, Chao Liang, Zhiyao Chen, Bingjie Zou, Qinxin Song, and Guohua Zhou

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2. A Simplified Protocol for Preparing Pyrosequencing Templates Based on LATE-PCR Using Whole Blood as Starting Material Directly

??????????? Yunlong Liu, Haiping Wu, Hui Ye, Zhiyao Chen, Qinxin Song, Bingjie Zou, Jianzhong Rui, and Guohua Zhou

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3. Improvement of LATE-PCR to Prepare Pyrosequencing Template

??????????? Qinxin Song, Huiyong Yang, Bingjie Zou, Hideki Kambara, and Guohua Zhou

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4. Pyrosequencing Templates Generated by Nicking PCR Products wil%