Affinity Chromatography Methods and Protocols [Hardcover]

The aim of this edition is to introduce the beginner to the basics of affinity chromatography and provide practical knowledge for the development of affinity separation protocols. Affinity Chromatography: Methods and Protocols, Third Edition guides readers through new state of the art protocols, molecular modelling, and the study of ligand-target interactions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Affinity Chromatography: Methods and Protocols, Third Edition is designed as a useful resource for those interested in the rapid and quantitative isolation of biomolecules with high purity.

1.????? Affinity Chromatography: A Historical

David S. Hage and Ryan Matsuda

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Part I: General Modes and Ligands of Affinity Chromatography

2.????? Protein Purification by Aminosquarylium Cyanine-Affinity Chromatography

V?nia C. Gra?a, Fani Sousa, Paulo F. Santos and Paulo S. Almeida

3.????? One Step Purification of Phosphinothricin Acetyltransferase using Reactive Dye Affinity Chromatography?

Cunxi Wang, Thomas C. Lee, Kathleen S. Crowley, and Erin Bell

4.????? Antibody Purification from Human Plasma by Metal-Chelated Affinity Membranes

Handan Yavuz, Nilay Bereli, Fatma Y1lmaz, Canan Armutcu and Adil Denizli

5.????? Specific Recognition of Supercoiled Plasmid DNA by Affinity Chromatography using a Synthetic Aromatic Ligand

Catarina Caramelo-Nunes and C?ndida T. Tomaz

6.????? Cell Affinity SeparatiolSd