Arthritis Research: Volume 2: Methods and Protocols [Paperback]

Arthritis Research: Methods and Protocols is a compendium of data pertinent to the methods and protocols that have contributed to recent advances in molecular medicine in general, but to the molecular basis of rheumatic disease in particular. These volumes details novel technologies, some of which are still evolving and whose impacts are yet to be determined. Leaders in the field contribute to cover such exciting and cutting edge topics as imaging and immunohistochemistry, analysis of cartilage and bone catabolism, immunobiology, and cell trafficking.

In Volume 1, authors discuss synovial joint morphology, histopathology, and immunohistochemistry, cartilage matrix and bone biology, and cell trafficking, migration and invasion. Volume 2 is broken up into sections that cover immunobiology and T cells, animal models of arthritis, and applications of new technologies, such as Differential Display Reverse Transcription-Polymerase Chain Reaction (DDRT-PCR), to define novel therapeutic targets. Both volumes combine to produce a concise set of protocols condensing decades of experience and expertise.

Arthritis Research: Methods and Protocols will be a valuable tool for basic research investigators, clinician scientists, pathologists, immunologists, and biochemists looking to stay current in their fields.

Immunobiology.- Phenotypic Analysis of B-Cells and Plasma Cells.- Detection of Antigen Specific B-Cells in Tissues.- Single Cell Analysis of Synovial Tissue B-Cells.- Tracking Antigen Specific CD4+ T-Cells With Soluble MHC Molecules.- Analysis of Antigen Reactive T-Cells.- Identification and Manipulation of Antigen Specific T-Cells with Artificial Antigen Presenting Cells.- Analysis of Th1/Th2 T-Cell Subsets.- Analysis of the T-Cell Receptor Repertoire of Synovial T-Cells.- The Assessment of T-Cell Apoptosis in Synovial Fluid.- Assays of T-Cell Contact Dependent Monocyte-Macrophage Functions.- Phenotypic and FunctionallÃ3