This volume focuses and describes tools, assays, and techniques used to enhance the understanding of the role of auxins and cytokinins. The chapters in this book cover topics such as: microbial manipulation of auxin and cytokinins in plants; interplay between auxin and cytokinin and its impact on MAPK; H2O2 production in Arabidopsis leaves; crosstalk between jasmonate and auxin in plant stress responses of roots; and high-throughput protoplast trans-activation (PTA) screening. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Auxins and Cytokinins in Plant Biology: Methods and Protocols is a valuable resource to plant scientists, graduate and under graduate students in addressing their biological questions relevant to the functional implications of auxin and cytokinins.
Preface...
Table of Contents...
Contributing Authors...
- Methodological Advances in Auxin and Cytokinin Biology
Andrej Hurn? and Eva Benkov?
- Analytical Determination of Auxins and Cytokinins
Petre I. Dobrev, Kl?ra Hoyerov?, and Jan Petr?aek
- Manipulation of Auxin and Cytokinin Balance During the Plasmodiophora brassicae: Arabidopsis thaliana Interaction
Jutta Ludwig-M?ller, Susann Auer, Sabine J?lke, and Sabine Marschollek
- Microbial Manipulation of Auxin and l(