The BAM Complex: Methods and Protocols [Paperback]

This volume is comprised of a collection of experimental protocols for common techniques and strategies used to study the biogenesis of b-barrel outer membrane proteins in Gram-negative bacteria. The BAM Complex: Methods and Protocols guides readers through methods on the function of the BAM complex, the roles played by each of the individual components, the expression and purification of the components, crystallization and structure determination of the components, and how the individual Bam components may assemble into a functional complex. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, The BAM Complex: Methods and Protocols will serve as an invaluable reference for those interested in studying the BAM complex.

The ?-barrel Assembly Machinery Comple.- Yeast Mitochondria as a Model System to Study the Biogenesis of Bacterial ?-barrel Proteins.- Experimental Methods for Studying the BAM Complex in Neisseria meningitides.- Heat Modifiability of Outer Membrane Proteins from Gram-Negative Bacteria.- The Role of a Destabilized Membrane for OMP Insertion.- Treponema pallidum in Gel Microdroplets: A Method for Topological Analysis of BamA (TP0326) and Localization of Rare Outer Membrane Proteins.- Analyzing the Role of Periplasmic Folding Factors in the Biogenesis of OMPs and Members of the Type V Secretion System.- An In Vitro Assay for Substrate Translocation by FhaC in Liposomes.- Measuring Cell-cell Binding Using Flow-Cytometry.- Methods to Characterize Folding and Function of BamA Crossl