The Blood-Brain Barrier: Methods and Protocols [Hardcover]

This detailed volume features techniques to explore the complex interface that separates the systemic circulation from the central nervous system, known as the blood-brain barrier (BBB). Beginning with an introduction to its physiology, the book continues with sections on using pluripotent stem cells in models of the BBB, co-culture, permeability and transwell models, microfluidic and chip models, as well as models to study specific BBB pathologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cells of the Blood-Brain Barrier: An Overview of the Neurovascular Unit in Health and Disease.- In Vitro Models of the Blood-Brain Barrier.- In Vitro Models of the Human Blood-Brain Barrier Based on Human Induced Pluripotent Stem Cells: Opportunities and Challenges.- Induced Pluripotent Stem Cell (iPSC)-Derived Endothelial Cells to Study BacterialBrain Endothelial Cell Interactions.- An hiPSC-Derived In Vitro Model of the Blood-Brain Barrier.- A Three-Dimensional Brain-on-Chip Using Human iPSC-Derived GABAergic Neurons and Astrocytes.- An Improved In Vitro Porcine Blood-Brain Barrier Model for Permeability Screening and Functional Studies.- Establishment of an In Vitro Model of Human Blood-Brain Barrier to Study the Impact of Ischaemic Injury.- A Novel Dynamic Human In Vitro Model for Studying the Blood-Brain Barrier.- A Dynamic, In Vitro BBB Model to Study the Effects of Varying Levels of ShealÃ3