Calcium-Binding Proteins of the EF-Hand Superfamily: From Basics to Medical Applications [Hardcover]

This detailed volume explores protocols for studying the many facets of Ca²⁺-imaging, Ca²⁺-signaling, and Ca²⁺-binding along with background information on the principles and application of these techniques. The content of the book delves into 48 chapters including subjects such as data analysis and modern technologies to study calcium-binding and signaling in cells, the superfamily of calcium-binding proteins characterized by the EF-hand structural motif, as well as their use as diagnostic and prognostic biomarkers in Laboratory Medicine and novel therapeutic drug targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Using Data Mining to Explore Calmodulin Bibliography.- Deep Two-Photon Imaging In Vivo with a Red-Shifted Calcium Indicator.- High Resolution Calcium Imaging Method for Local Calcium Signaling.- Measurement of Contractility and Calcium Release in Cardiac Spheroids.- Simultaneous Recording of Subcellular Ca²⁺ Signals from the Cytosol and Sarco/Endoplasmatic Reticulum: Compartmentalized Dye Loading, Imaging, and Analysis.- The Use of Complementary Luminescent and Fluorescent Techniques for Imaging Ca²⁺ Signalling Events during thl|