Calorimetry [Hardcover]

Calorimetry, the latest volume in the Methods in Enzymology series continues the legacy of this premier serial with quality chapters authored by leaders in the field. Calorimetry is a highly technical experiment and it is easy for new practioners to get fooled into interpreting artifacts as real experimental results.? This volume will guide readers to get the most out of their precious biological samples and includes topics on specific protocols for the types of studies being conducted as well as tips to improve the data collection. Most importantly, the chapters will also help to identify pitfalls that need to be avoided to ensure that the highest quality results are obtained.

• Contains timely contributions from recognized experts in this? rapidly changing field
• Provides specific protocols and tips to improve data collection and ensure the highest quality results are obtained
• Covers research methods in calorimetry, and includes sections on topics such as differential scanning calorimetry of membrane and soluble proteins in detergents

1.?Isothermal Titration Calorimetry Measurements of Metal Ions Binding to Proteins
Colette F. Quinn ,Margaret C. Carpenter, Molly L. Croteau and Dean E. Wilcox
2.?Assessing Coupled Protein Folding and Binding through Temperature- Dependent ?? Isothermal Titration Calorimetry
Debashish Sahu, Monique Bastidas, Chad W. Lawrence, William G. Noid,and Scott? A. Showalter
3.?Fragment-Based Screening for Enzyme Inhibitors Using Calorimetry
Michael I. Recht, Vicki Nienaber and Francisco E. Torres
4.?Measuring Multivalent Binding Interactions by Isothermal Titration Calorimetry
Tarun K. Dam,? Melanie L Talaga, Ni Fan and Curtis F. Brewer
5.?Calorimetric and Spectroscopic Analysis of the Thermal Stability of Short Duplex DNA-Containing Sugarló•