cAMP Signaling: Methods and Protocols [Paperback]

This volume discusses the latest techniques used by researchers to measure cAMP activity at the cell population, whole cell, and subcellular level. The techniques covered in the book address questions related to cAMP compartmentalization, which look at relevant protein-protein interactions that increase the spatial and temporal resolution of cAMP signals detection, and that can help in the integration of the increasingly complex information that is becoming available in this field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and comprehensive, cAMP Signaling: Methods and Protocols, Second Edition is a valuable resource for scientists and researchers who are interested in learning more about this important and developing field. 

Chapter 7 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Real-Time Measurements of Intracellular cAMP Gradients using FRET-Based cAMP Nanorulers.- Assaying Protein Kinase A Activity using a FRET-Based Sensor Purified from Mammalian Cells.- MultiFRET: A Detailed Protocol for High-Throughput Multiplexed Ratiometric FRET.- Photoactivated Adenylyl Cyclases as Optogenetic Modulators of Neuronal Activity.- Imaging the cAMP Signaling Microdomain of the Primary Cilium using Targeted FRET-Based Biosensors.- Methods to Assess Phosphodiesterase and/or Adenylyl Cyclase Activity via Heterologous Expression in Fission Yeast.- Time-Domain Fluorescence Lifetime Imaging of cAMP Levels with EPAC-Based FRET Sensors.-&nblS&