This volume presents state of the art of methods that can be useful for both basic and translational researchers to conduct chemoprevention preclinical studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cancer Chemoprevention: Methods and Protocols aims to ensure successful results in the further study of this vital field.
1. Controlled Delivery of Chemopreventive Agents by Polymeric Implants
Farrukh Aqil and Ramesh C. Gupta
?
2. Use Of Buffy Coat Mirna Profiling for Breast Cancer Prediction in Health Women
Sara Donzelli ,Giovanni Blandino, and Paola Muti
?
3. Micrornas in Cancer Chemoprevention: Method to Isolate them from Fresh Tissues
Federica Ganci and Giovanni Blandino
?
4. Application of RNA-Seq Technology in Cancer Chemoprevention
Frauke Goeman and Maurizio Fanciulli
?
5. Detection of Circulating Tumor DNA in the Blood of Cancer Patients: An Important Tool in Cancer Chemoprevention
Peter Ulz, Martina Auer, and Ellen Heitzer
?
6. The Methylated DNA Immunoprecipitation (Medip) to Investigate the Epigenetic Remodeling in Cell Fate Determination and Cancer Development.
Silvia Masciarelli, Teresa Bellissimo, Ilaria Iosue, and Francesco Fazi
?
7. LC-MS-Based Metabolomic Investigation Of ChemopreventivePhytochemical-Elicited Metabolic Events