Cell-Cell Interactions: Methods and Protocols [Paperback]

In?the second edition of Cell-Cell Interactions: Methods and Protocols,?expert researchers in the field detail a collection of protocols to examine interactions between cells. These include protocols that focus on both?in vivo?and?in vitro?methods-based approaches. New updated chapters also bring together many currently used assays in examining cell-cell interactions and the biological consequences of those interactions. Written in the highly successful?Methods in Molecular Biology??series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. ?Authoritative and Practical,?Cell-Cell Interactions: Methods and Protocol, Second Edition?is a valuable resource for researchers who are already involved in the cell-cell interaction field, and also for those?that are new to the area.

Proteomics Analysis of Contact-initiated Eph Receptor-ephrin Signaling.- Control of Vascular Tube Morphogenesis and Maturation in 3D Extracellular Matrices by Endothelial Cells and Pericytes.- Analyzing Cell-cell Interactions in 3-dimensional Adhesion Assays.- Production of Spontaneously Beating Neonatal Rat Heart Tissue for Calcium and Contractile Studies.- Paracrine Communication Between Mechanically Stretched Myocytes and Fibroblasts.- Assessing Blood Brain Barrier Function using in vitro Assays.- Methods to Assess Tissue Permeability.- In vivo Quantification of Metastatic Tumor Cell Adhesion in the Pulmonary Microvasculature.- Cell Membrane Vesicles as a Tool for the Study of Direct Epithelial-stromal Interaction: Lessons from CD147 .- Microencapsulation of Stem Cells to Study Cellular Interactions.- Cell-surface Protein-protein Interaction Analysis with Time-resolved FRET and Snap-tag Technologieslãx