In?the second edition of Cell-Cell Interactions: Methods and Protocols,?expert researchers in the field detail a collection of protocols to examine interactions between cells. These include protocols that focus on both?in vivo?and?in vitro?methods-based approaches. New updated chapters also bring together many currently used assays in examining cell-cell interactions and the biological consequences of those interactions. Written in the highly successful?Methods in Molecular Biology??series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. ?Authoritative and Practical,?Cell-Cell Interactions: Methods and Protocol, Second Edition?is a valuable resource for researchers who are already involved in the cell-cell interaction field, and also for those?that are new to the area.
This book details protocols to examine interactions between cells, including those that focus on both in vivo and in vitro methods-based approaches. It provides step-by-step detail essential for reproducible results and contains key implementation advice.
1. Proteomics Analysis of Contact-initiated Eph Receptor-ephrin Signaling
Claus Jorgensen and Alexei Poliakov
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2. Control of Vascular Tube Morphogenesis and Maturation in 3D Extracellular Matrices by Endothelial Cells and Pericytes
George E. Davis, Dae Joong Kim, Chun-Xia Meng, Pieter R. Norden, Katherine R. Speichinger, Matthew T. Davis, Annie O. Smith, Stephanie L.K. Bowers, and Amber N. Stratman
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3. Analyzing Cell-cell Interactions in 3-dimensional Adhesion Assays
Stepl#
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