Circulating MicroRNAs Methods and Protocols [Paperback]

microRNAs (miRNAs) are small non-coding RNAs that regulate various biological phenomena, such as development and homeostasis. The dysregulation of miRNA leads to disease progression, particularly of cancer. In Circulating MicroRNAs: Methods and Protocols,  expert researchers in the field detail recent advances in the isolation, purification and analysis of circulating miRNAs from a variety of sources for research. The book is divided into three main topics. The first section involves the study of secretory miRNAs in cell-cell communication, and the second, the study of circulating miRNAs in body fluids. The last describes the novel techniques used to study circulating miRNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Circulating MicroRNAs: Methods and Protocols seeks to aid scientists in dealing with the recent advances of RNAi technology from the bench to the bedside.

1. Functional Analysis of Exosomal MicroRNA in Cell-Cell Communication Research

Nobuyoshi Kosaka, Yusuke Yoshioka, Keitaro Hagiwara, Naoomi Tominaga and Takahiro Ochiya

 

2.  Isolation of Extracellular Nanovesicle MicroRNA from Liver Cancer Cells in Culture 

Takayuki Kogure and Tushar Patel