Coupled Bioluminescent Assays: Methods, Evaluations, and Applications [Hardcover]

This book highlights the applications of coupled bioluminescence assay techniques to real-world problems in drug discovery, environmental and chemical analysis, and biodefense. It separates theoretical aspects from the applied sections in a clear and readable way. Coupled Bioluminescent Assays, explains the uses of CB technologies across drug discovery to analyze toxicity, drug receptors, and enzymes. It covers applications in environmental analysis and biodefense, including cytotoxicity, fertilizer and explosives analysis, and nerve agent and pesticide detection. This is the premier reference on coupled bioluminescent assays for chemists, biochemists, and molecular biologists.

Preface xiii

Part I Background to Coupled Bioluminescent Assays 1

1 Introduction 3

1.1 Introduction to Coupled Bioluminescent Assays, 3

1.2 Luminescent Technologies of the Life Sciences, 4

1.3 Varieties of Fluorometric Assays, 7

1.3.1 Time-Resolved Fluorescence, 8

1.3.2 Fluorescence Resonance Energy Transfer, 8

1.3.3 Fluorescence Polarization, 9

1.4 Chemiluminescence and Bioluminescence, 10

1.4.1 Chemiluminescence, 11

1.4.2 Bioluminescence, 13

1.5 Common Bioluminescence Systems, 14

1.5.1 Firefly Luciferase, 14

1.5.2 Renilla Luciferase, 16

1.5.3 Bacterial Luciferases, 18

1.5.4 Aequorin, 19

1.5.5 Other Luciferases and Photoproteins, 20

1.6 A Coupled Bioluminescent Reaction, 22

1.7 Summary, 23

2 Coupled Bioluminescent Reactions in Practice 24

2.1 Principles of Coupled Bioluminescent Reactions, 24

2.1.1 Requirements for Suló×