Developmental Toxicology: Methods and Protocols [Paperback]

The discipline of developmental toxicology is an integration of concepts, models, and methodologies based heavily on the superimposition of toxicology principles upon the science of developmental biology.? The science of developmental toxicology also borrows from other research areas that are concerned with regulation of cell growth, migration, differentiation and cell death, as such are central to the study of stem cells, cancer, and chronic diseases. In Developmental Toxicology: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study developmental toxicology highlighting the evolution of methods from classical teratology approaches to the dynamic, state-of-the-art molecular methods, systems biology, and next generation models and procedures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

?

Authoritative and practical, Developmental Toxicology: Methods and Protocols is a valuable resource to those planning experiments to investigate consequences of environmental, nutritional, or chemical effects caused during development.

Volume Overview - Introduction.-In Vivo Models of Developmental Toxicology.-Caenorhabditis Elegans as a Model in Developmental Toxicology.-Zebrafish Embryo Developmental Toxicology Assay.-Gene Knockdown by Morpholino-modified Oligonucleotides in the Zebrafish (Danio rerio) Model: Applications for Developmental Toxicology.-An Amphibian Model for Studies of Developmental Reproductive Toxicity.-Avian Models in Teratology and Developmental Toxicology.-Overview of In Vitro Models in Developmental Toxicololc.