Digital PCR: Methods and Protocols [Hardcover]

This volume explores and explains how digital PCRs (dPCRs) help in the study of numerous topics, such as infectious diseases, evolution of cancer and treatment responses, somatic mosaicism, genome editing and cell therapy, and food testing for GMOs and pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Digital PCR: Methods and Protocols is a valuable resource for specialists in various fields, including geneticists, neurologists, immunologists, oncologists, and researchers who are interested in environmental sciences.

Entering the Pantheon of 21st Century Molecular Biology Tools: A Perspective on Digital PCR.- Basic Concepts and Validation of Digital PCR Measurements.- Fundamentals of Counting Statistics in Digital PCR: I Just Measured Two Target Copies, What Does it Mean?.- Control Materials and Digital PCR Methods for Evaluation of Circulating Cell Free DNA Extractions from Plasma.- Multiplex Droplet Digital PCR Protocols for Quantification of GM Maize Events.- Using Droplet Digital PCR to Detect Coinfection of Human Herpesviruses 6A and 6B (HHV-6A and HHV-6B) in Clinical Samples.- Biomarkers in Cerebrospinal Fluid: Analysis of Cell-Free Circulating Mitochondrial DNA by Digital PCR.- Testing of General and Human-Associated Fecal Contamination in Waters.- Analyzing Copy Number Variation with Droplet Digital PCR.- Assessing HER2 Amplification in Plasma cfDNA.- Detection and Quantification of Mosaic Genomic DNA Variation in Primary l#¼