Embryonic Stem Cell Protocols: Volume II: Differentiation Models [Hardcover]

Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume two, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. The first volume, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells.?Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.Isolation and Maintenance.- Neural Stem Sphere Method.- Generation and Characterization of Oligodendrocytes From Lineage-Selectable Embryonic Stem Cells In Vitro.- Derivation and Characterization of Neural Cells From Embryonic Stem Cells Using Nestin Enhancer.- Optimized Neuronal Differentiation of Murine Embryonic Stem Cells.- Generation of Inner Ear Cell Types From Embryonic Stem Cells.- Derivation of Epidermal Colony-Forming Progenitors From Embryonic Stem Cell Cultures.- Directing Epidermal Fate Selection by a Novel Co-Culture System.- Signaling in Embryonic Stem Cell Differentiation.- In Vitro Generation of T Lymphocytes From Embryonic Stem Cells.- The Role of Hex in Hemangioblast and Hematopoietic Development.- Generation of Osteoblasts and Chondrocytes From Embryonic Stem Cells.- Analysis of Embryonic Stem Cell-Derived Osteogenic Cultures.- Generation of Chondrocytes From Embryonic Stem Cells.- Derivation and Characterization of Chondrocytes From Embryonic Stem Cells In Vitro.-Generation and Characterization of Cardiomyocytes Under Serum-Free Conditions.- Analysis l3J