Experiments in Plant Tissue Culture [Paperback]

This new edition of a highly successful book has been completely revised and updated, and features new illustrations and experiments.The new edition of a guide to the essentials of plant tissue culture has been completely revised and updated. The organization has been improved, and new illustrations as well as experiments on such topics as potato callus formation and embryo culture have been added.The new edition of a guide to the essentials of plant tissue culture has been completely revised and updated. The organization has been improved, and new illustrations as well as experiments on such topics as potato callus formation and embryo culture have been added.In this third edition the authors have written a guide to the essentials of plant tissue culture. The book takes the reader through a graded series of experimental protocols and provides an introductory review of each topic. There are discussions of aseptic techniques, and nutritional components of media. Subsequent chapters cover such topics as callus formation, organogenesis, xylem cell differentiation, root cultures, cell suspensions, micropropagation by buds, somatic embryogenesis, protoplast culture and fusion, secondary metabolite production, and quantitation of procedures. A glossary, commercial sources of supplies, and the formulations of culture media are also included. This new edition has been completely revised and updated. The organization has been improved, and new illustrations have been added, together with new experiments on such topics as potato callus formation, and embryo culture. This book will be useful to graduate students and professionals in plant science, forestry, agronomy, and horticulture.Part I. Background Information: 1. Culture of plant cells, tissues, and organs; 2. Laboratory facilities; 3. Aseptic techniques; 4. Media composition and preparation; Part II. Experimental: Callus and Callus-Derived Systems: 5. Initiation and maintenance of callus; 6. Organogenesis; 7. Cell suspenl#/