Functional Genomics [Hardcover]

This collection of robust, readily reproducible methods for microarray-based studies includes expert guidance in the optimal data analysis and informatics. On the methods side are proven techniques for monitoring subcellular RNA localization en masse, for mapping chromosomes at the resolution of a single gene, and for surveying the steady-state genome-wide distribution of DNA binding proteins in vivo. For those workers dealing with massive data sets, the book discusses the methodological aspects of data analysis and informatics in the design of microarray experiments, the choice of test statistic, and the assessment of observational significance, data reduction, and clustering.Fabrication of cDNA Microarrays.- Nylon cDNA Expression Arrays.- Plastic Microarrays.- Preparing Fluorescent Probes for Microarray Studies.- Escherichia coli Spotted Double-Strand DNA Microarrays.- Isolation of Polysomal RNA for Microarray Analysis.- Parallel Analysis of Gene Copy Number and Expression Using cDNA Microarrays.- Genome-wide Mapping of Protein-DNA Interactions by Chromatin Immunoprecipitation and DNA Microarray Hybridization.- Statistical Issues in cDNA Microarray Data Analysis.- Experimental Design to Make the Most of Microarray Studies.- Statistical Methods for Identifying Differentially Expressed Genes in DNA Microarrays.- Detecting Stable Clusters Using Principal Component Analysis.- Clustering in Life Sciences.- A Primer on the Visualization of Microarray Data.- Microarray Databases. ....a good book, and it will be useful to microarray workers in their effots. - Journal of Chemical NeuroanatomyAs high-throughput methods become more available and routine, experimentalists and theoreticians must be prepared to take advantage of the analytical power these methods offer. In Functional Genomics: Methods and Protocols, leading experimentalists-many pioneers in the field-describe in step-by-step detail robust methods for microarray-based studies and provide expert guidance in optl3Á