Functional Genomics [Hardcover]

This collection of robust, readily reproducible methods for microarray-based studies includes expert guidance in the optimal data analysis and informatics. On the methods side are proven techniques for monitoring subcellular RNA localization en masse, for mapping chromosomes at the resolution of a single gene, and for surveying the steady-state genome-wide distribution of DNA binding proteins in vivo. For those workers dealing with massive data sets, the book discusses the methodological aspects of data analysis and informatics in the design of microarray experiments, the choice of test statistic, and the assessment of observational significance, data reduction, and clustering.This collection of robust, readily reproducible methods for microarray-based studies includes expert guidance in the optimal data analysis and informatics. On the methods side are proven techniques for monitoring subcellular RNA localization en masse, for mapping chromosomes at the resolution of a single gene, and for surveying the steady-state genome-wide distribution of DNA binding proteins in vivo. For those workers dealing with massive data sets, the book discusses the methodological aspects of data analysis and informatics in the design of microarray experiments, the choice of test statistic, and the assessment of observational significance, data reduction, and clustering.Fabrication of cDNA MicroarraysCharlie C. Xiang and Michael J. BrownsteinNylon cDNA Expression ArraysGeorge Jokhadze, Stephen Chen, Claire Granger, and Alex ChenchikPlastic Microarrays: A Novel Array Support Combining the Benefits of Macro- and Microarrays, Alexander MunishkinKonrad Faulstich, Vissarion Aivazachvili, Claire Granger, and Alex ChenchikPreparing Fluorescent Probes for Microarray StudiesCharlie C. Xiang and Michael J. BrownsteinEscherichia coli Spotted Double-Strand DNA Microarrays: RNA Extraction, Labeling, Hybridization, Quality Control, and Data ManagementArkady B. Khodursky, Jonathan A. Bernstein, Brian lƒ"