G Protein-Coupled Receptor--Protein Interactions [Hardcover]

This exciting volume elucidates how GPCRs organize signal transduction and control intracellular activities. Chapters written by the recognized expert in each respective topic offer state-of-the-art updates on the principles and technology, as well as stepwise protocols for methods currently applied to the analysis of protein-receptor interactions.Foreword (Robert J. Lefkowitz).

Series Preface (David R. Sibley).

Preface (Susan R. George & Brian F. O'Dowd).

Contributors.

1. Purification of the G Protein-Coupled Receptor Rhodopsin for Structural Studies (David Salom, Ning Li, Li Zhu, Izabela Sokal and Krzysztof Palczewski).

2. Cooperativity in Ligand Binding to Deduce Receptor/Receptor Interaction (Meritxell Vivo and Philip G. Strange).

3. Analysis of Receptor-Receptor Interaction Using Time-Resolved Fluorescence Resonance Energy Transfer (Douglas Ramsay and Graeme Milligan).

4. Real-Time Imaging of GPCR-Medicated Arresting Translocation as a Strategy to Evaluate Receptor-Protein Interactions (Robert H. Oakley, Larry S. Barak and Marc G. Caron).

5. Localization of G Protein Coupled Receptors and Interacting Proteins in Membrane Microdomains (K. Saidas Nair, Vijaya Narayanan and Vladlen Z. Slepak).

6. Identification of Peptide Ligand Binding Domains Within G Protein-Coupled Receptors Using Photoaffinity Labeling (Maoqing Dong and Laurence J. Miller).

7. Analysis in Real-Time of the Assembly and Disassembly of Soluble G Protein-Coupled Receptor-G Protein Complexes (Seam M. Biggs, Peter I. Simons, Anna Waller, Terry Founts, Tione Buranda, Eric R. Prossnitz and Larry A. Sklar).

8. Approaches to Studying Membrane Protein Oligomerization: Use of Cysteine Cross-Linking Strategies to Analyze Receptor-Receptor Interactions (Jonathan A. Javitch, Wen Guo and Marisa E. Ring).