Gel-Free Proteomics Methods and Protocols [Hardcover]

Proteomics by means of mass spectrometry has rapidly changed the way that we analyze proteomes. Gel-Free Proteomics: Methods and Protocols addresses contemporary methods for gel-free proteome research with a special focus on differential analysis and protein modifications. Divided into twenty-five chapters, this detailed volume meticulously describes vital procedures needed to perform gel-free proteomics, ranging from sample preparation, isotope labeling for differential proteomics, enrichment technologies for modified proteins and peptides, and bioinformatics. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

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Authoritative and easily accessible, Gel-Free Proteomics: Methods and Protocols serves as a timely resource for both professionals and novices pursing research in this critical field.

1. Mass Spectrometry-driven Proteomics: An Introduction

??????????? Kenny Helsens, Lennart Martens, Jo?l Vandekerckhove, and Kris Gevaert

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2. Metabolic Labeling of Model Organisms using Heavy Nitrogen (15N)

??????????? Joost W. Gouw, Bastiaan B.J. Tops, and Jeroen Krijgsveld

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3. Trypsin-catalyzed Oxygen-18 Labeling for Quantitative Proteomics