Germline Development in the Zebrafish: Methods and Protocols [Paperback]

This volume details a wide range of methods, ranging from beginner through advanced, used to

further study zebrafish and fish germline.. Chapter guide readers through cultivating and manipulating germ cells, imaging of germline processes and the molecular analysis of their, protein, and RNA.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Germline Development in the Zebrafish: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Separation of Oocyte and Follicle Layer for Gene Expression Analysis in Zebrafish.- The Xenopus Oocyte as an Expression System for Functional Analyses of Fish Aquaporins.- Computer-Assisted Sperm Analysis to Test Environmental Toxicants.- Cryopreservation and Transplantation of Spermatogonial Stem Cells.- Masculinization of Zebrafish through Partial Depletion of Primordial Germ Cells by Injecting Diluted Morpholino Oligonucleotides into Embryos.- Chemical Genetics: Manipulating the Germline with Small Molecules.- In vitro Induction of Teleost PGCs.- Applying Rho Pathway Inhibitors to Investigate Germ Plasm Localization.- Cryopreservation of Pooled Sperm Samples.- Quantifying Tissue Tension in the Granulosa Layer after Laser Surgery.- In-vivo and Ex-vivo CT Imaging of Zebrafish Gonads.- Live and Time-lapse Imaging of Early Oogenesis and Meiotic Chromosomal Dynamics in Cultured Juvenile Zebrafish Ovaries.- Detection of the Polar Body after Fertilization.- Ll#'