Handbook of Industrial Cell Culture Mammalian, Microbial, and Plant Cells [Hardcover]

A diverse team of researchers, technologists, and engineers describe, in simple and practical language, the major current and evolving technologies for improving the biocatalytic capabilities of mammalian, microbial, and plant cells. The authors present state-of-the-art techniques, proven methods, and strategies for industrial screening, cultivation, and scale-up of these cells, and describe their biotech and industrial uses. Special emphasis is given to the solving critical issues encountered during the discovery of new drugs, process development, and the manufacture of new and existing compounds. Other topics include recombinant protein expression, bioinformatics, high throughput screening, analytical tools in biotechnology, DNA shuffling, and genomics discovery.A diverse team of researchers, technologists, and engineers describe, in simple and practical language, the major current and evolving technologies for improving the biocatalytic capabilities of mammalian, microbial, and plant cells. The authors present state-of-the-art techniques, proven methods, and strategies for industrial screening, cultivation, and scale-up of these cells, and describe their biotech and industrial uses. Special emphasis is given to the solving critical issues encountered during the discovery of new drugs, process development, and the manufacture of new and existing compounds. Other topics include recombinant protein expression, bioinformatics, high throughput screening, analytical tools in biotechnology, DNA shuffling, and genomics discovery.Part I. Mammalian Cell CultureAnalysis and Manipulation of Recombinant Glycoproteins Manufactured in Mammalian Cell CultureNigel JenkinsGenetic Approaches to Recombinant Protein Production in Mammalian CellsPeter P. Mueller, Dagmar Wirth, Jacqueline Unsinger, and Hansj?rg HauserProtein Expression Using Transgenic AnimalsWilliam H. Velander and Kevin E. van CottMammalian Cell Culture: Process Development ConsiderationsSteven Rose, Thomas Black, anlC%