HDAC/HAT Function Assessment and Inhibitor Development: Methods and Protocols [Paperback]

This detailed collection covers how the biological functions of histone deacetylases (HDACs) and histone acetyltransferases (HATs) can be detected in various experimental settings, both in vivo and in vitro. The book also covers the generation and specificity of deacetylase inhibitors and how such agents can be used to test experimental hypotheses. Written for the popular Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.

Detection of Autophagy Induction After HDAC Inhibitor Treatment in Leukemic Cells.- Assessment of HDACi-Induced Protein Cleavage by Caspases.- Assessment of HDACi-Induced Cytotoxicity.- Analysis of HDACi-Induced Changes in Chromosomal Passenger Complex Localization.- Analysis of Histone Deacetylase-Dependent Effects on Cell Migration Using the Stripe Assay.- Using Histone Deacetylase Inhibitors to Analyze the Relevance of HDACs for Translation.- Effects of HDACi on Immunological Functions.- DNA Fiber Spreading Assay to Test HDACi Effects on DNA and Its Replication.- Assessing the Effect of Class I Histone Deacetylase Activity on DNA Double-Strand Break Repair by Homologous Recombination.- Establishment and Characterization of Long-Term Cultures Derived from Primary Acute Myeloid Leukemia Cells for HDAC Inhibitor Research.- Assessing the Histone Deacetylase Activity of SIRT6 in Primary Murine Hepatocytes Via Proximity Ligation Assay.- Purification and Analysis of Male Germ Cells from Adlów