Heterologous Expression of Membrane Proteins: Methods and Protocols [Hardcover]

This detailed volume encompasses chapters from leadingexperts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functionalproduction and stabilization of recombinant integral membrane proteins (IMPs). Membraneproteins play a key role in numerous pathologies such as cancer, cysticfibrosis, epilepsy, hyperinsulinism, and Alzheimers disease, yet studies onthese and other disorders are hampered by a lack of information about theproteins involved. This book sets out to aid researchers in rectifying thissituation. Written for the highly successful Methods in Molecular Biology series, chapters include introductionsto their respective topics, lists of the necessary materials and reagents,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.

Authoritative and up-to-date, HeterologousExpression of Membrane Proteins: Methods and Protocols, Second Editionserves as an ideal guide for scientists attempting to delve deeper into the myriadunique IMP structures.

Cell-Free Production of Membrane Proteins in Escherichia coli Lysates for Functionaland Structural Studies.- Membrane Protein Production in the Yeast, S. cerevisiae.- Membrane ProteinsProduction in Escherichia coli:Protocols and Rules.- Codon Optimizing for Increased Membrane ProteinProduction: A Minimalist Approach.- Generation of Tetracycline-InducibleMammalian Cell Lines by Flow Cytometry for Improved Overproduction of MembraneProteins.- Membrane Protein Production in Lactococcus lactis for FunctionalStudies.- Expression ofViral Envelope Glycoproteins in Drosophila melanogaster S2 Cells.- Leishmania tarentol? as a Promising Toolfor Expressing Polytopic and Multi-Transmembrane Spans Eukaryotic MembraneProteins:l£'