This detailed volume provides a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and exploring innovative engineering of E. coli. The topics are largely grouped under four parts: high-throughput cloning, expression screening, and optimization of expression conditions, protein production and solubility enhancement, case studies to produce challenging proteins and specific protein families, as well as applications of E. coli expression. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Heterologous Gene Expression in E. coli: Methods and Protocols serves molecular biologists, biochemists and structural biologists, those in the beginning of their research careers to those in their prime, to give both an historical and modern overview of the methods available to express their genes of interest in this exceptional organism.
Recombinant Protein Expression in E. coli: A Historical Perspective.- N- and C-Terminal Truncations to Enhance Protein Solubility and Crystallization: Predicting Protein Domain Boundaries with Bioinformatics Tools.- Harnessing the Profinity eXactTM System for Expression and Purification of Heterologous Proteins in E. coli.- ESPRIT: A Method for Defining Soluble Expression Constructs in Poorly Understood Gene Sequences.- Optimizing Expression and Solubility of Proteins in E. coli Using Modified Media and Induction Parameters.- Optimization of Membrane Protein Production Using Titratable Strlsq
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