HIV Reservoirs: Methods and Protocols [Paperback]

This book details the development of methods and models to study the HIV-1 viral reservoir with the ultimate goal of achieving a functional cure of HIV infection. Chapters are divided into six parts covering cell lines, in vitro and ex vivo primary cell models of persistent infection, in vitro and ex vivo tissue-derived models, infected animal models human immune cells, methods of detection and analysis of the reservoir, and current approaches to achieve either a functional cure or cART-free long-term remission. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, HIV Reservoirs: Methods and Protocols provides a comprehensive, updated collection of state-of-art methodologies and models to tackle the HIV-1 viral reservoir.

Jurkat-derived (J-Lat, J1.1 and Jurkat E4) and CEM-derived T cell lines (8E5, ACH-2) as Models of Reversible Proviral Latency.- U1 and OM10.1. Myeloid Cell Lines as Surrogate Models Of Reversible Proviral Latency.- An In Vitro System to Model the Establishment and Reactivation of HIV-1 Latencyin primary human CD4+ T cells.- The cultured T_CM model of HIV latency.- A Reliable Primary Cell Model for HIV latency: The QUECEL (Quiescent Effector Cell Latency) Method.- TGF-b signaling supports HIV latency in a Memory CD4+ T cell based in vitro model.p Flow Cytometry Sorting of Memory CCR6⁺CD4⁺