Hypertension: Methods and Protocols [Hardcover]

A collection of new and essential molecular techniques for cardiovascular research. These readily reproducible methods range widely from producing congenic, consomic, transgenic, and knockout models of hypertension to the gene transfer of specific genetic material using nonviral (polymers, liposomes, and antisense agents) and adenoviral vectors. Additional techniques described include single nucleotide polymorphism (SNP) genotyping, RNA interference, microarray analysis, pharmacogenetics, and pharmacogenomics for the genetic dissection of hypertension, as well as a practical method for deriving cardiomyocytes from embryonic stem cells that would serve as replacement cells for those damaged by hypertension or heart attack. The book offers both novice and experienced hypertension researchers an indispensable collection of readily reproducible techniques for successful research, work that has already dramatically improved the outlook for hypertensive patients, and promises much future success.Models Of Hypertension.- Congenic/Consomic Models of Hypertension.- Mouse Knockout Models of Hypertension.- Production of Transgenic Models in Hypertension.- Measuring Blood Pressure in Small Laboratory Animals.- Assessment Of Free Radicals In Endothelial Dysfunction.- Analysis of Superoxide Anion Production in Tissue.- Measurement of Vascular Reactive Oxygen Species Production by Chemiluminescence.- A Guide to Wire Myography.- Nucleic Acid Techniques.- Selection of Candidate Genes in Hypertension.- Genome-Wide Scanning With SSLPs in the Rat.- Extraction of RNA From Cells and Tissue.- Single-Strand Conformational Polymorphism Analysis.- Single-Nucleotide Polymorphism Genotyping for Disease Association Studies.- Denaturing High-Performance Liquid Chromatography Using the WAVE DNA Fragment Analysis System.- TaqMan Real-Time PCR Quantification Conventional and Modified Methods.- Generation of High-Sensitivity Antisense cDNA Probes by Asymmetric PCR.- New Advances in Microarrays FindilÓy