Immunochemistry of Solid-Phase Immunoassay [Hardcover]

Immunochemistry of Solid-Phase Immunoassay fills a nich? in the field of immunoassay and immunology. Although solid-phase immunoassay constitutes a major technology in biology and medicine, there is no comprehensive source devoted to the immunochemical principles involved. As a result, this book will benefit students, technicians, and researchers who use this technology, as well as immunodiagnostic and biotech companies who develop the technology. The book is not a methods manual; instead, it incorporates the concepts, data, and opinions of more than 25 investigators working in this field. Topics discussed include: the chemistry of solid-phases, the behavior or antibodies and antigens on solid phases, membrane solid-phases, reaction kinetics, antigen quantitation, enzyme systems, photophysics, immunochemical considerations in data analyses, multianalyte assays and occupancy concepts, antibody quantitation, streptavidin, a review of data analysis software, and solid-phase peptide immunoassay.General Principles: Perspectives, Configurations, and Principles (J.E. Butler). The Chemical Properties of Solid-Phases and Their Interaction With Proteins (P.J. Tarcha). Immunochemical Considerations in the Analysis of Data from Non-Competitive Solid-Phase Immunoassays (J.H. Peterman). Photophysics of Heterogeneous Immunoassays (O.S. Khahil). Enzyme Systems and Enzyme Conjugates for Solid-Phase ELISA (T.T. Ngo). Practical Applications: Competitive, Noncompetitive and Multianalyte Immunoassays (R.P. Ekins). Antigen Quantification: Measurement of Multivalent Antigens by Solid-Phase Immunoassay (R.G. Hamilton). Immunoblotting: Membrane Filters as the Solid Phase for Immunoassays (W.R. Brown, S.E>Dierks, J.E. Butler, and J.M. Gershoni). Quantitation of Specific Antibodies: methods of Expression, Standards, Solid-Phase Considerations, and Specific Applications (J.E>Butler and R.G. Hamilton). Critical Issues and Special Topics: The Specificity of Monoclonal Antibodies for Enzymes in ls0