Immunogenetics: Methods and Protocols [Hardcover]

This open access book explores techniques for working in the field of immunogenetics, i.e. fundamental and translational research into the adaptive immune receptor repertoire. Many chapters are dedicated to lab protocols, bioinformatics, and immunoinformatics analysis of high-resolution immunome analysis, exemplified by numerous applications. Additionally, the newest technological variations on these protocols are discussed, including non-amplicon, single-cell, and cell-free strategies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. The Advent of Precision Immunology: Immunogenetics at the Center of Immune Cell Analysis in Health and Disease.- Next-Generation Sequencing-Based Clonality Detection of Immunoglobulin Gene Rearrangements in B-Cell Lymphoma.- One-Step Next Generation Sequencing of Immunoglobulin and T-Cell Receptor Gene Recombinations for MRD Marker Identification in Acute Lymphoblastic Leukemia.- Immunoglobulin/T-Cell Receptor Gene Rearrangement Analysis Using RNA-Seq.- Minimal Residual Disease Analysis by Monitoring Immunoglobulin and T-Cell Receptor Gene Rearrangements by Quantitative PCR and Droplet Digital PCR.- Quality Control for IG/TR Marker Identification and MRD Analysis.- cfDNA-Based NGS IG Analysis in Lymphoma.- Targeted Locus AmplificatlÓy