Shotgun Proteomics Methods and Protocols [Hardcover]

Shotgun Proteomics: Methods and Protocols serves as a vital collection of protocols through which thousands of proteins can be simultaneously identified, quantified and characterized in a high throughput manner. Beginning with the history of proteomics centered on the vital role of mass spectrometry in its development, this detailed volume continues with chapters on sample pre-fractionation, in vivo and in vitro stable isotope labeling, label-free proteomics, informatics, protein-protein interactions, targeted proteomics and post-translational modifications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Practical and comprehensive, Shotgun Proteomics: Methods and Protocols is an ideal and up-to-date guide for researchers seeking to understand the proteome of any given species.

Part I: Introduction

1. Survey of Shotgun Proteomics

??????????? Fabio Cesar Sousa Nogueira and Gilberto B. Domont?

Part II: Sample Pre-Fractionation

2. LC-MALDI-TOF/TOF for Shotgun Proteomics

??????????? Patricia Fern?ndez-Puente, Jes?s Mateos, Francisco J. Blanco and Cristina Ruiz-Romero

3. Fully Automatable Multidimensional Reversed-Phase Liquid Chromatography with Online Tandem Mass Spectrometry

??????????? Maggie P.Y. Lam, Henry Law, Quan Quan, Yun Zhao, and Ivan K. Chu

?4. GeLC-MS/MS Analysis of Complex Protein Mixtures

??????????? Monika Dzieciatkowska, Ryan Hill, and Kirk C. Hansen

5. IPG Strip-Based Peptide Fractionation for Shotgun Proteomics

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