Amyloid Proteins Methods and Protocols [Hardcover]

Amyloid diseases are characterized by the deposition of insoluble fibrous amyloid proteins. The word amyloid indicates a starch-like compound, and though a misnomer, continues to be the accepted term for this group of protein conformational disorders. The second edition? of Amyloid Proteins expands upon the previous edition with current, detailed protocols for the preparation of amyloid and its precursors, specific analytical methods for studying these proteins, cell culture models and assays for production of amyloid proteins, and protocols for amyloid extraction from tissue, its detection in vitro and in vivo, as well as nontransgenic methods for developing amyloid mouse models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Amyloid Proteins, Second Edition seeks to aid scientists in the amyloid field to establish new techniques in their laboratories.

Authoritative and practical, Amyloid Proteins, Second Edition seeks to aid scientists in the amyloid field to establish new techniques in their laboratories.

In its new edition, this Methods in Molecular Biology book offers protocols for preparing amyloid and its precursors, cell culture models, extraction from tissue, detection in vitro and in vivo and nontransgenic methods for developing amyloid mouse models.

Part I. In Vivtro Models and Assays

1. Rapid Generation of Dityrosine Cross-linked A? Oligomers via Cu-redox Cycling