Cellular Regulation by Protein Phosphorylation [Paperback]

Proceedings of the NATO Advanced Study Institute on Cellular Regulation by Protein Phosphorylation held at Chateau La Londe les Maures (France), September 5-15, 1990I. Background and General Introduction.- Signal Integration in Phosphorylase Kinase.- Physical Approaches to Conformation and Assembly of Biological Macromolecules.- A Vibrational Raman Spectroscopic Study of Myosin and Myosin-Vanadate Interactions.- Phosphorylation and the Frequency Encoding of Signal-induced Calcium Oscillations.- II. Methodology.- Determination of Phosphorylated Amino Acids in Protein Sequences.- Back-Phosphorylation - a Sensitive Technique to Study Protein Phosphorylation in the Intact Heart.- Thermal Transitions in Cardiac Troponin and Its Subunits.- Dynamic Phosphorylation of a Small Chloroplast Protein Exhibiting So Far Undescribed Labelling Properties.- Identification of Phosphorylation Sites in the Nicotinic Acetylcholine Receptor by Edman Degradation and Mass Spectroscopy LC/MS and LC/MS/MS.- A Sensitive Colorimetric Assay for Protein Phosphatase Activity.- III. Structure - Function Relationship.- Protein Kinase Structure and Function: cAMP-Dependent Protein Kinase.- Expression in E. Coli of Mutated R Subunits of the cAMP-Dependent Protein Kinase From Dictyostelium Discoideum.- Expression of the -Subunit of Phosphorylase b Kinase in E. Coli.- Conformational and Shape Changes Associated with cAMP-Dependent Protein Kinase.- Crystallographic Studies of the Catalytic Subunit of cAMP-Dependent Protein Kinase.- 6-Phosphofructo 2-Kinase/Fructose 2,6-Bisphosphatase: Kinetic Changes Induced by Phosphorylation.- Enzymes Involved in the Reversible Phosphorylation in Microvessels of the Brain.- Covalent Modification of Creatine Kinase by ATP; Evidence for Autophosphorylation.- Two Adjacent Phosphoserines in Bovine, Rabbit and Human Cardiac Troponin I.- Characterisation of Thylakoid Membrane Protein Kinase by Affinity and Immunological Methods.- IV. CA 2+ and Cyclic Nucleotide-Independent Pl39