Drug Metabolism and Transport Molecular Methods and Mechanisms [Paperback]

A compendium of proven experimental approaches and strategies for studying the bioactivation, detoxification, tissue distribution, and elimination of xenobiotics in the metabolism and/or transport of various chemicals. The authors address several of the major drug metabolizing systems, including the cytochrome P450 family, flavin-containing monooxygenases, glutathione, S-transferase, glucuronidation, N-acetylation, and sulfotransferases. Additional chapters present novel approaches to the study of: signaling pathways in the regulation of drug metabolism enzymes, how the modulation of thiols and other low molecular-weight cofactors can alter drug metabolism, and how modulation of drug metabolism pathways can influence antiviral therapy.A compendium of proven experimental approaches and strategies for studying the bioactivation, detoxification, tissue distribution, and elimination of xenobiotics in the metabolism and/or transport of various chemicals. The authors address several of the major drug metabolizing systems, including the cytochrome P450 family, flavin-containing monooxygenases, glutathione, S-transferase, glucuronidation, N-acetylation, and sulfotransferases. Additional chapters present novel approaches to the study of: signaling pathways in the regulation of drug metabolism enzymes, how the modulation of thiols and other low molecular-weight cofactors can alter drug metabolism, and how modulation of drug metabolism pathways can influence antiviral therapy.Renal Cytochrome P450s and Flavin-Containing Monooxygenases: Potential Roles in Metabolism and Toxicity of 1,3-Butadiene, Trichloroethylene, and TetrachloroethyleneAdnan A. ElfarraExperimental Approaches for the Study of Cytochrome P450 Gene RegulationHollie I. Swanson, Susan D. Kraner, Soma S. Ray, Martin Hoagland, Earl D. Thompson, Xinyu Zheng, and Yanan TianInsulin and Growth Factor Signaling: Effects on Drug-Metabolizing EnzymesSang K. Kim, Kimberley J. Woodcroft, and Raymond F. NovakCatalytic Functl3µ