Duchenne Muscular Dystrophy: Methods and Protocols [Hardcover]

This volume explores experimental approaches used to study Duchenne muscular dystrophy (DMD), an X-linked degenerative skeletal muscle disease caused by mutations in the dystrophin gene. Including the latest progress and scientific achievements, the book covers recent discoveries achieved through in vivo gene editing which have proven to be promising in restoring dystrophin expression, at least in ameliorating skeletal muscle symptoms, and the contents focus on Omics techniques in gene expression, protein expression, miRNAs, and long non-coding RNA analysis, as well as experimental studies of the structural/functional changes affecting the skeletal and cardiac muscles and ongoing preclinical studies and clinical trials. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshootingand avoiding known pitfalls.?

An Overview of Recent Therapeutics Advances for Duchenne Muscular Dystrophy.-?Clinical Manifestations and Overall Management Strategies for Duchenne Muscular Dystrophy.- Cardiac Involvement in Duchenne Muscular Dystrophy and Related Dystrophinopathies.- Characterization of the Inflammatory Response in Dystrophic Muscle Using Flow Cytometry.- Imaging Analysis of the Neuromuscular Junction in Dystrophic Muscle.- System Biology Approach: Gene Network Analysis for Muscular Dystrophy.- Proteomic Profiling of the Dystrophin-Deficient Brain.- Probing the Pathogenesis of Duchenne Muscular Dystrophy Using Mouse Models.- Exon Skipping Therapy Using Phosphorodiamidate Morpholil(