Light Spectroscopy [Paperback]

Spectrophotometry and spectrofluorometry are core techniques used throughout biology and medicine. These techniques evolve continuously and this book provides information on the latest advances in spectroscopic methods. Light Spectroscopy begins by descrAbbreviations -- Preface -- PART 1: BASIC PRINCIPLES AND METHODS -- 1. Principles of Spectrophotometry -- The nature of spectrophotometry -- Interaction of light with matter -- Absorption lines and absorption bands -- Absorption spectra -- Quantitative aspects of absorption -- Light emission in solution - fluorescence -- Fluorescence spectra and the quantitation of fluorescence -- Light scattering revisited -- 2. What to Look at -- Chromophores -- Fluorophores -- Effect of environment on absorption -- Effect of environment on fluorescence -- Sensitivity and detection limits -- Intrinsic and extrinsic probes -- Indicator molecules -- 3. Spectrophotometer Design -- Components -- Light sources -- Wavelength selection -- The sample compartment -- Detectors -- Varying the wavelength (scanning) -- Data capture -- 4. Geometry, Light Paths and Beam Splitting -- Sources of error in absorbance measurements -- The single beam spectrophotometer -- The double beam spectrophotometer -- Variations on a theme -- The dual wavelength spectrophotometer -- Sources of error in fluorescence measurements -- The spectrofluorometer -- The dual wavelength fluorometer -- Measurements on opaque samples -- 5. Measuring Absorbance and Fluorescence -- Calibrating the instrument -- Choosing the cuvette -- Preparing the sample -- PART 2: TECHNIQUES AND APPLICATIONS -- 6. Measuring an Absorption Spectrum -- Introduction -- Calibrating the instrument -- Selecting a sample -- Choice of operating conditions -- Determining a baseline -- Interpreting spectra -- Working without oxygen -- How to improve resolution -- 7. Measuring a Fluorescence Emission Spectrum -- Types of fluorescence spectrum -- Calibrating the fluorometer -- Preparing the sample -- Sellã+