Membrane Proteins Folding, Association, and Design [Hardcover]

Focusing on model systems for the study of structure, folding, and association in the membrane, Membrane Proteins: Folding, Association, and Design presents an overview of methods that can be applied to these intricate systems. The volume is divided into four detailed sections, covering association of transmembrane helices, interactions with the lipid bilayer, NMR methods, as well as a variety of engineering approaches.? Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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Authoritative and practical, Membrane Proteins: Folding, Association, and Design serves as an ideal guide for researchers reaching for the tantalizing possibility of designing novel membrane proteins with tailored functionality.This book focuses on model systems for the study of structure, folding, and association of proteins in the membrane. It presents laboratory-ready protocols and features key tips and implementation advice to ensure successful results.

Part I: Association of Transmembrane Helices

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1. Use of Thiol-Disulfide Exchange Method to Study Transmembrane Peptide Association in Membrane Environments

??????????? Lidia Cristian and Yao Zhang

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2. Measurement of Transmembrane Peptide Interactions in Liposomes Using F?rster Resonance Energy Transfer (FRET)

??????????? Ambalika Khadria and Alessandro Senes

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3. Measuring Transmembrane Helix Interaction Strengths in Lipid Bilayers Using Steric Trapping

??????????? Heedeok Hong, Yu-Chu Chang, and James U. Bowie

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