Methods in Membrane Lipids [Paperback]

Methods in Membrane Lipids presents a compendium of methodologies for the study of membrane lipids, varying from traditional lab bench experimentation to computer simulation and theoretical models. This volume provides a comprehensive set of techniques for studying membrane lipids, and their interactions with other membrane lipids, with membrane proteins, and with exogenous agents such as anesthetics. The progression of chapters increases in complexity, initially presenting introductory concepts and methods for the study of simple structures and interactions, then moving to the study of more complex structures and interactions, including: behaviors of lipids in water systems and phases; membrane lipid movements and diffusion; properties of lipids in mono and bilayers; complex lipid domains; and membrane-lipid interaction with proteins and drugs. Each chapter is written by an internationally recognized expert in the field.

A Glance at the Structural and Functional Diversity of Membrane Lipids.- Membrane Lipid Polymorphism.- Acrylodan-Labeled Intestinal Fatty Acid-Binding Protein to Measure Concentrations of Unbound Fatty Acids.- Measuring Molecular Order and Orientation Using Coherent Anti-Stokes Raman Scattering Microscopy.- Preparation of Oriented, Fully Hydrated Lipid Samples for Structure Determination Using X-Ray Scattering.- Nuclear Magnetic Resonance Investigation of Oriented Lipid Membranes.- Molecular Dynamics Simulations as a Complement to Nuclear Magnetic Resonance and X-Ray Diffraction Measurements.- Use of Inverse Theory Algorithms in the Analysis of Biomembrane NMR Data.- Statistical Thermodynamics Through Computer Simulation to Characterize Phospholipid Interactions in Membranes.- Fluorometric Assay for Detection of Sterol Oxidation in Liposomal Membranes.- Fluorescence Detection of Signs of Sterol Superlattice Formation in Lipid Membranes.- Differential Scanning Calorimetry in the Study of Lipid Phase Transitions in Model and BiologilÓÙ