Mobile Genetic Elements: Protocols and Genomic Applications [Hardcover]

Transposable elements are used as genetic tools for dissecting the function of a specific gene and elaborating on mechanisms leading to genetic change and diversity, and the evolutionary impact of mobile DNA on the biology and evolution of organism. In, Mobile Genetic Elements: Protocols and Genomic Applications, Second Edition gathers a wide array of strategies and protocols for identifying transposable elements (TEs) and their evolutionary derivatives, and for studying genome structure, function, and evolution. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

?

Through and intuitive Mobile Genetic Elements: Protocols and Genomic Applications, Second Edition aids scientists in the continuing study of in silico tools, strategies and protocols that are complementary of those described in the first volume.

Transposable Elements as Tools for Reshaping the Genome: it's a Huge World After All !.-Using and Understanding RepeatMasker.-Roadmap for Annotating Transposable Elements in Eukaryote Genomes.-To? Detect? and? to? Analyze Sequence Repeats Whatever? their? Origin.-Exploring Bacterial Insertion Sequences with ISfinder: Objectives, Uses and Future? Developments.-Methods and Software in NGS for TE Analysis.-The Application of LTR Retrotransposons As Molecular Markers in Plants.-Individual Analysis of Transposon Polymorphisms by AFLP.-Construction of a Library of Random Mutants in the Spirochete Leptospira Biflexa using a Mariner Transposon.-Ac-Ds Solutions for Rice Insertion Mutagenesis.-Engineering the Caenorhabditis elegans Genome by Mos1 Induced Transgene-Inslƒ