Skeletal Muscle Development [Hardcover]

This detailed volume presents a wide range of techniques that go beyond the standard assays typically used to assess Myogenesis. The content included addresses assays to analyze skeletal muscle gene expression, proliferating muscle cells, the process of myoblast fusion, muscle development (in vivo), as well as muscle repair. Written for the highly successful?Methods in Molecular Biology?series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?

Authoritative and practical,?Skeletal Muscle Development?serves as an ideal guide to the study of this highly complex yet carefully regulated process.>

Part I: Skeletal Muscle Gene Expression

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1. Bisulfite Sequencing for DNA Methylation Analysis of Primary Muscle Stem Cells

??????????? Kohei Miyata, Masashi Naito, Tomoko Miyata, Sho Mokuda, and Hiroshi Asahara

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2. Whole Genome Chromatin IP-Sequencing (ChIP-Seq) in Skeletal Muscle Cells

??????????? Karl Kamhei So, Xianlu Peng, Hao Sun, and Huating Wang

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3. Analysis of RNA Expression in Adult Zebrafish Skeletal Muscle

??????????? Tamar E. Sztal, Peter D. Currie, and Robert J. Bryson-Richardson

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4. Chick Somite Electroporation for Gene Function Studies

??????????? Muhammad Abu-Elmagd

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Part II: Assays for Proliferating Skeletal Muscle Cells

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5. Targls!