This volume expands on the previous edition with a more extensive look at molecular motors and their roles in muscle contractions, vesicle transport, flagellar beating, chromosome segregation, and DNA replication and repair. The chapters in this book are divided into three parts: Part One looks at membrane motors, such as the bacterial flagellar rotary motor; Part Two discusses cytoskeletal motors, such as kinesin and myosin; and Part Three talks about nucleic acid motors, such as DNA polymerases, helicases, and nucleosome remodelers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive,
Molecular Motors: Methods and Protocols, Second Edition is a valuable resource for (bio)physicists and molecular/cellular biologists whose research delves into the mechanisms at work in cells and the motors which power them.
Preface&
Table of Contents&
Contributing Authors&
1. Cellular and Nuclear Forces: An Overview
Bidisha Sinha, Arikta Biswas, and Gautam V. Soni
Part I Membrane Motors
2. The Bacterial Flagellar Rotary Motor in Action
Nicholas J.S. Delalez and Judith P. Armitage
3. Purification and Reconstitution of Ilyobacter tartaricus ATP Synthase
Ganna O. Krasnoselska and Thomas Meier
Part II Cytoskeletal Motlóú
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