This volume providesa sound basis for the molecular investigation of NLR function in health anddisease. Chapters focus on of innate immune receptors, atypical inflammasomes, biochemical and novel bioluminescencetechniques for the measurement of IL-1b,bioluminescent probe, biochemical and microscopytechniques, techniques to measure caspase-1 activation, cellfree systems for the study of inflammasome function, and inflammasomeactivation. Written in the highly successful Methodsin Molecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.
Authoritativeand cutting-edge, NLR Proteins: Methodsand Protocols aims to ensure successful results in the further study ofthis vital field.
Innate Immune Receptors.- Atypical Inflammasomes.- Assessment of Inflammasome Activation By Cytokine And Danger Signal Detection.- Investigating IL-1? Secretion Using Real-Time Single-Cell Imaging.- Measuring IL-1? Processing By Bioluminescence Sensors I: Using a Bioluminescence Resonance Energy Transfer Biosensor.- Measuring IL-1? Processing By Bioluminescence Sensors II: The Igluc System.- Assessing Extracellular ATP as Danger Signal In Vivo: The Pmeluc System.- Measuring NLR Oligomerization I: Size Exclusion Chromatography, Co-Immunoprecipitation and Cross-Linking.- Measuring NLR Oligomerization II: Detection of ASC Speck Formation by Confocal Microscopy and Immunofluorescence.- Measuring NLR Oligomerization III: Detection of NLRP3 Complex by Bioluminescence Resonance Energy Transfer.- Measuring NLR Oligomerization IV: Using F?rster Resonance Energy Transfer (FRET)-Fluorescence Lifetime Imaging Microscopy (FLIM) to Determine the Close Proximity Of Inflammasome Componentsl£6
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