Adult Stem Cells: Methods and Protocols [Paperback]

This volume contains a collection of protocols from some of the major laboratories involved in stem cell research across the world. The research discussed in this book covers topics such as: isolating, characterizing and expanding dental pulp stem cells; manipulating the proliferative potential of cardiomyocytes by gene transfer; isolation of stromal stem cells from adipose tissue; noninvasive assessment of cell fat and biology in transplanted mesenchymal stem cells; and cell-free therapy for organ repair. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Adult Stem Cells: Methods and Protocols is a valuable resource for helping researchers transform the study of stem cells into an industrialized process that will supply patients with efficient, safe, and cost-effective cell treatments.

A Simple Protocol to Isolate, Characterize and Expand Dental Pulp Stem Cells.- Derivation of Mesenchymal Stem cells from Embryonic Stem Cells: A Non-Variable and Inexhaustive Source of Adult Stem Cells.- Compact Bone-Derived Multipotent Mesenchymal Stromal Cells (MSCs) for the Treatment of Sjogrens-Like Disease in NOD Mice.- Manipulating the Proliferative Potential of Cardiomyocytes by Gene Transfer.- Assessment of Energy Metabolic Changes in Adipose Tissue-Derived Stem Cells.- Isolation and In Vitro Characterization of Epidermal Stem Cells.- Endothelial Progenitor Cells: Procedure for Cell Isolation and Characterization.- Therapeutic Application of Placental Mesenchymal Stem Cells Reprogrammed Neurospheres in Spinal Cord Injury of SCID.- Therapeutic Application of Human WhartonlÓC