Lysosomes Methods and Protocols [Paperback]

This volume provides laboratory protocols essential for studies on lysosomal biology. Chapters aim to guide researchers in their exploration of lysosomes, both under normal conditions and in pathological processes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Lysosome: Methods and Protocols aims to provided protocols that will guide and inspire further research and generate new insights into this fascinating organelle.

1.      SILAC-Based Comparative Proteomic Analysis of Lysosomes from Mammalian Cells Using LC-MS/MS
Melanie Thelen, Dominic Winter, Thomas Braulke, and Volkmar Gieselmann

 

2.      Quantitative Profiling of Lysosomal Lipidome by Shotgun Lipidomics
 Mesut Bilgin, Jesper Nylandsted, Marja J??ttel?, and Kenji Maeda

 

3.      Analysis of N- And O-Glycosylation of Lysosomal Glycoproteins
Elmira Tokhtaeva, Olga A. Mareninova, Anna S. Gukovskaya, and Olga Vagin

 

 

5.      Microscopic Analysis of Lysosomal Membrane Permeabilization
Ana Maria Vilamill Giraldo, Karin ?llinger, and Vesa Lls‚