Ebolaviruses: Methods and Protocols [Paperback]

This vital book collects key methods that have supported advancements in the field of ebolavirus molecular biology given the pressing need for the advancement of techniques for diagnostics, the development of vaccines and antivirals, and for furthering our understanding of ebolavirus biology. After an introduction, the volume delves into protocols for studying ebolavirus molecular biology under biosafety level 2 conditions, studying infectious ebolaviruses under biosafety level 4 conditions in vitro as well as in vivo, and working with ebolaviruses in the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Marburg and Ebola Viruses: A Look Back and Lessons for the Future.- 40 Years of Ebolavirus Molecular Biology: Understanding a Novel Disease Agent through the Development and Application of New Technologies.- Ebolavirus: An Overview of Molecular and Clinical Pathogenesis.- Production of Filovirus Glycoprotein-Pseudotyped Vesicular Stomatitis Virus for Study of Filovirus Entry Mechanisms.- Lentiviral Vectors Pseudotyped with Filoviral Glycoproteins.- Modeling Ebola Virus Genome Replication and Transcription with Minigenome Systems.- Quantification of RNA Content in Reconstituted Ebola Virus Nucleocapsids by Immunoprecipitation.- Modeling Ebolavirus Budding with Virus-Like Particles.- Modeling the Ebolavirus Life Cycle with Transcription and Replication-Competent Virus-Like Particle Assays.- Assays to Measure SupplãÎ