Host-Bacteria Interactions: Methods and Protocols [Paperback]

Host-Bacteria Interactions: Methods and Protocols details cutting edge protocols that cover aspects of the investigation of host bacteria interactions using mammalian and novel non mammalian infection models, cell biology, OMICS and bacterial genetics. Chapters focus on techniques that can be used to investigate different aspects of the physiopathology of bacterial infections, from the whole animal to tissue, cellular and molecular level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Host-Bacteria Interactions: Methods and Protocols provide researchers with a comprehensive account of the practical steps necessary for carrying out each protocol successfully.

Galleria mellonella as an Infection Model for Select Agents.- Drosophila as a Model for Intestinal Infections.- Zebrafish Embryos as a Model to Study Bacterial Virulence.- Studying Host-pathogen Interaction Events in Living Mice Visualized in Real Time using Biophotonic Imaging.- Intravital Two photon Imaging to Understand Bacterial Infections of the Mammalian Host.- Cre Reporter Assay for Translocation (CRAfT), A Tool? for the Study of Protein Translocation into Host Cells.- Detection of the Interaction Between Host and Bacterial Proteins: Eukaryotic Nucleolin Interacts with Francisella Elongation Factor Tu.- Hijacking the Host Proteasome for the Temporal Degradation of Bacterial Effectors.-?Life Cell Imaging of Phosphoinositide Dynamics during Legionella Infection.- Investigating Interference with Apoptosis Induction by Bacterial Proteins.- Bimolecular FluoreslƒD