This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.
Plant DNA Repair Pathways and their Applications in Genome Editing.- Rapid Screening of CRISPR/Cas9-Induced Mutants using the ACT-PCR Method.- Decoding Sanger-Sequencing Chromatograms from CRISPR-Induced Mutations.- Creating Large Chromosomal Deletions in Rice using CRISPR/Cas9.- A Multiplexed CRISPR-Cas9 Editing System Based on the Endogenous tRNA Processing.- A Single Transcript CRISPR-Cas9 System for Multiplex Genome Editing in Plants.- CRISPR-Act2.0: An Improved Multiplexed System for Plant Transcriptional Activation.- Generating Photoperiod-Sensitive Genic Male Sterile Rice Lines with CRISPR-Cas9.- Knocking Out MicroRNA Genes in Rice with CRISPR/Cas9.- An Agrobacterium-Mediated CRISPR-Cas9 Platform for Genome Editing in Maize.- Fluorescence Marker-Assisted Isolation of Cas9-Free and CRISPR-Edited Arabidopsis Plants.- Creating Targeted Gene Knock-Outs in Brassica oleracea using CRISPR/Cas9.- Applicationlăg
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