The purpose of this book is to provide information which supports the fact that rat hybridomas are no more difficult to develop than mouse hybridomas. This is the first book devoted to the development of rat hybridomas. It includes theories, step-by-step techniques, ingredients and apparatus. The focus of this work is on the antibody repertoire, the unique biological properties of rat immunoglobulins, the one-step purification procedure by immunoaffinity chromatography, the absence of C-type particles, and the easy production of large amounts of ascitic fluid containing rat MAb. This rare publication is an absolute must for all scientists using MAbs and those interested in the fields of immunology, biotechnology, and biochemistry.
Introduction
Part I IMMUNOCYTOMAS in LOUVAIN (LOU) RATS.
Chapter 1
Rat Immuoglobulins.
Chapter 2
The LOUVAIN (LOU) Rats.
Chapter 3
Rat Immunocytomas (IR).
Part II PRODUCTION of RAT-RAT HYBRIDOMAS.
Chapter 4
Fusion Cell Lines.
Chapter 5
Immunization of Rats.
Chapter 6.I
Fusion Procedure.
Chapter 6.II
Rat Hybridoma Prodcution: Scanning electron Microscopy of a Fusion Experiment
Chapter 7
Selective Growth of Hybrids.
Chapter 8
Cloning.
Chapter 9.I
Screening of Monoclonal Antibodies Directed Against Soluble Antigens
Chapter 9.II
Screening of Hybrid Supernatants for Monoclonal Antibodies Against Cellular Antigens
Chapter 10
Isotyping of Rat Monoclonal Antibodies.
Chapter 1lóc
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