RNA Detection and Visualization: Methods and Protocols [Paperback]

With its complex and extensively regulated metabolism, the study of the RNA lifecycle demands tools that allow for the localization of RNAs to be observed either in an in situ setting or, preferably, under in vivo conditions.? In RNA Detection and Visualization: Methods and Protocols, the best and brightest investigators provide an up-to-date and in-depth description of basic methods and protocols used for detecting and visualizing mRNAs in both fixed and live cells, from bacteria to mammals.? For novices and experts alike, this mix of classic in situ hybridization and advanced live imaging techniques, cell fractionation and affinity purification procedures, and bioinformatics tools gives researchers the most complete and extensive array of research aids possible. As a volume written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.

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Single Molecule Imaging of RNA In situ.- FISH and Immunofluorescence Staining in Chlamydomonas.- High Resolution Fluorescent In situ Hybridization in Drosophila.- Localization and Anchorage of Maternal mRNAs to Cortical Structures of Ascidian Eggs and Embryos Using High Resolution In situ Hybridization.- Visualization of mRNA Localization in Xenopus Oocytes.- Visualization of mRNA Expression in the Zebrafish Embryo.- High-Resolution Fluorescence In situ Hybridization to Detect mRNAs in Neuronal Compartments lÓÙ