Clinical Proteomics: Methods and Protocols [Hardcover]

This detailed volume explores techniques in clinical proteomics, an emerging discipline aimed at deciphering the molecular mechanisms underlying the progression of diseases and at identifying new biomarkers and therapeutic targets to expand the physicians toolbox for precision medicine-based patient care. From sample processing to multi-omics approaches, the book provides straight-forward protocols on a wide array of vital areas of study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Bile Processing Protocol for Improved Proteomic Analysis.- MS-Based Extracellular Vesicles (EVs) Analysis: An Application to Helminth-Secreted EVs.- Proteomic Profiling of Cerebrospinal Fluid by 16-Plex TMT-Based Mass Spectrometry.- Data-Independent Acquisition Mass Spectrometry-Based Deep Proteome Analysis for Hydrophobic Proteins from Dried Blood Spots Enriched by Sodium Carbonate Precipitation.- Sample Processing for Metaproteomic Analysis of Human Gut Microbiota.- Mass Spectrometry-Based Analytical Strategy for Single Cell Proteomics.- Lysine Acetylation Stoichiometry Analysis at the Proteome Level.- Implementation of Clinical Phosphoproteomics and Proteomics for Personalized Medicine.- pH/Acetonitrile-Gradient-Reversed-Phase Fractionation of Enriched Hyper-Citrullinated Library in Combination with LCMS/MS Analysis for Confident Identification of Citrullinated Peptides.- Purification of HLA Immunopeptidomes fromlÓC