This volume highlights natural products, molecular methods for identifying, and current trends in designing non-natural natural products. Chapters guide readers through protocols on heterologous expression techniques, gene disruption, modified pathway regulators, and in-vitro studies. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Engineering Natural Product Biosynthesis: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
Chapter 13 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
A bioinformatics workflow for investigating fungal biosynthetic gene clusters.- Investigating Fungal Biosynthetic Pathways Using Heterologous Gene Expression - Aspergillus oryzae as a heterologous host.- Investigating Fungal Biosynthetic Pathways Using Heterologous Gene Expression: Aspergillus nidulans as a Heterologous Host.- Investigating Fungal Biosynthetic Pathways Using Heterologous Gene Expression: Fusarium sp. as a Heterologous Host.- Heterologous Expression of Fungal Biosynthetic Pathways in Aspergillus nidulans using episomal vectors.- Targeted Genetic Engineering via Agrobacterium-mediated transformation in Fusarium solani.- Investigating Fungal Biosynthetic Pathways Using Pichia pastoris as a Heterologous Host.- Evolutionary genome mining for the discovery and engineering of natural product biosynthesis.- Indlƒ
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